SILAC-labeled proteins in ribosome samples
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https://www.omicsdi.org/dataset/pride/PXD011032
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资源简介：
we developed a novel method combining axon-pSILAC17 with axonal ribosome purification. In this method, we first labeled newly synthesized proteins with heavy amino acids (AAs) in somaless axons cultured in a Boyden chamber. Approximately 2000 eyes were cultured for each experiment to obtain sufficient axonal material. Then, axonal ribosomes were purified using a sucrose cushion and ultracentrifugation, followed by identification of newly synthesized proteins with mass spectrometry.
创建时间：
2019-12-09
