SILAC-labeled proteins in ribosome samples
收藏NIAID Data Ecosystem2026-03-11 收录
官方服务：
资源简介：
we developed a novel method combining axon-pSILAC17 with axonal ribosome purification. In this method, we first labeled newly synthesized proteins with heavy amino acids (AAs) in somaless axons cultured in a Boyden chamber. Approximately 2000 eyes were cultured for each experiment to obtain sufficient axonal material. Then, axonal ribosomes were purified using a sucrose cushion and ultracentrifugation, followed by identification of newly synthesized proteins with mass spectrometry.
创建时间：
2019-12-09
