Effect of bone marrow-specific aryl hydrocarbon receptor (Ahr) gene knockout on expression profiles of hematopoietic stem cells from young and old mice.

As part of a study of the role of the aryl hydrocarbon receptor (Ahr) in maintenance and senescence of hematopoietic stem cells (HSC), global gene expression profiling was done with HSC isolated from bone marrow restricted conditional Ahr-knockout and AhR floxed mice. HSC from young-adult (8 wk old) cAhR-KO mice had changes in expression of many genes related to HSC maintenance, consistent with the phenotype observed in Ahr-KO mice. Aged cAhR-KO mice (18 months old) also displayed alterations in peripheral white blood cell counts, serial repopulation potential and levels of ROS in bone marrow cells, consistent with previous observations on the role of AhR in the hematopoietic system. 22 samples: 5 young Ahr knockout, 6 old Ahr knockout, 5 young floxed Ahr, 6 old floxed Ahr本研究旨在探究芳香烃受体（aryl hydrocarbon receptor, Ahr）在造血干细胞（hematopoietic stem cells, HSC）维持与衰老过程中的作用，我们对从骨髓限制性条件性Ahr基因敲除（cAhR-KO）及AhR flox小鼠骨髓中分离得到的HSC进行了全基因表达谱分析。年轻成年（8周龄）cAhR-KO小鼠的HSC中，诸多与HSC维持相关的基因表达发生改变，这与在Ahr基因敲除小鼠中观察到的表型一致。18月龄的老年cAhR-KO小鼠同样表现出外周白细胞计数、连续重建造血潜能及骨髓细胞活性氧（reactive oxygen species, ROS）水平的异常，这与此前关于AhR在造血系统中作用的研究结果相符。本数据集共包含22个样本：5例年轻Ahr基因敲除组、6例老年Ahr基因敲除组、5例年轻AhR flox组及6例老年AhR flox组。