H9c2 cell response after retinoic acid stimuli

During culture, H9c2 cells acquire a myotubule phenotype where a critical component is the inclusion of retinoic acid (RA). The results from some authors on H9c2 suggested that thousands of genes respond to RA stimuli, while other authors report hundreds of genes responding to RA over different cell types. We investigated the response to RA in H9c2 cells controlling for culture time.在细胞培养过程中，H9c2细胞会形成肌管表型，而该表型的关键诱导条件为培养基中添加视黄酸（retinoic acid, RA）。部分针对H9c2细胞的研究结果显示，数千个基因会对视黄酸刺激产生应答；而另有学者在不同细胞类型中开展的研究则报告称，仅数百个基因会响应视黄酸刺激。本研究通过控制培养时长这一变量，探究了H9c2细胞对视黄酸的应答情况。