Neutrophil aging exacerbates high fat diet induced metabolic alterations

In this work, we aimed to characterize how a high-fat diet affects neutrophil plasticity and aging in the bone marrow, as well as their infiltration into inflamed metabolic tissues. <br> For the metabolic responses, we performed shotgun proteomics as reported here in physiology (wild-type mice, WT) and in mice with constitutively aged neutrophils (MRP8 driven conditional deletion of CXCR4; herein CXCR4fl/flCre+) or with constitutively fresh neutrophils (MRP8 driven conditional deletion of CXCR2; CXCR2fl/flCre+) following feeding with a High Fat Diet (HFD) (45% Kcal from fat) which promote the development of obesity.