Multifaceted rewiring of the hepatic lipidome during proliferation and carcinogenesis - PART 1 (Liver Regeneration)

The scope of this project is to study, using state-of-the-art systems biology approaches integrating the changes in metabolomics, lipidomics and transcriptomics, changes in hepatocytes' metabolism occurring in liver regeneration. We focused on altered metabolic pathways including lipogenesis, fatty acid desaturation, and generation of phosphatidylcholine (PC) occurring in the liver following partial hepatectomy (PH) or carbon-tetrachloride (CCl4) injection. Male C57BL/6J mice were challenged with A) IP injection of 2ul/g body weight of olive oil (OO) or CCl4:olive oil [1:1 (v/v)] mix; Mice were culled 3 days after IP injection. B) Partial Hepatectomy; Mice were culled 3 days after surgery.