Figure 2: Characterization of Contractile Function and Calcium Transients in hiPSC-CMs

<b>(A)</b> No difference in maximum averaged normalized shortening in day 30 WT-CMs and FCM-CMs. <b>(B, C)</b> Impaired maximum fractional shortening is evident in day 50 FCM-CMs compared with age-matched WT-CMs. (N = 3 WT lines and N = 3 FCM lines, n &gt; 10 cells for each day 30 line; Kruskal-Wallis test, p &gt; 0.05) (N = 3 WT lines and N = 3 FCM lines, n &gt; 21 for each day 50 line; Kruskal-Wallis test, p &lt; 0.05; *p &lt; 0.05 with 2-tailed Mann-Whitney <i>U</i> test). <b>(D)</b> Normalized averaged calcium transients in day 50 human induced pluripotent stem cell–derived cardiomyocyte (hiPSC-CMs) are not significantly different. All cells paced at 1.5 Hz. Error bars indicate SEM. See Supplemental Videos 1 and 2. Abbreviations as in Figures 1 and 2.