Response of Rpp4-silenced plants to soybean rust. Glycine max

Experimental design: 1 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) 2 treatments: Rub inoculation with BPMV-VIGS construct targeting the LRR region of Rpp4 candidate gene. This BPMV construct and the plant tissue used for microarray was described in Meyer et al. (2009) Plant Physiol. 150:295-307. Control plants were inoculated with empty BPMV vector. At three weeks after BPMV inoculation, all plants were inoculated with Soybean rust (Phakopsora pachyrhizi) isolate LA04-1. LA04-1 is a single spore isolate maintained at the Foreign Disease Weed Science Research Unit. 3 replications 1 time point: 14 days after inoculation TOTAL: 5 Affymetrix GeneChip(R) Soybean Genome Arrays LA04-1 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Michelle Graham. The equivalent experiment is GM51 at PLEXdb.] Overall design: treatment: BPMV-LRR(2-replications); treatment: BPMV empty vector(3-replications)