Protocol for analyse of expression of IL-1 receptors.

Optimize protocol of sample preparation for determining the expression level of IL-1RI and IL-1RII using the QuantiBRITE PE kit, as well as estimating quantitatively the number of membrane-bound receptors on various subpopulations of immunocompetent cells from healthy donors. Conclusion: We have designed an optimal protocol for estimating the expression level of membrane-bound IL-1Rs, with the aid of QuantiBRITE PE calibration beads. Determination of the mean number of membrane-bound molecules of IL-1Rs using this protocol enables us to obtain precise and reproducible data that are required for the more complete and informative estimation of receptor expression levels.