Pyridinoline content and telopeptide hydroxylation of bone collagen.

Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP), were measured in bone collagen. Listed are the HP and LP contents (moles/mole of collagen), and the molar ratio (HP/LP) for Lepre1H662A/H662A and Lepre1+/+ mice showing a significant increase in the HP/LP ratio in the Lepre1H662A/H662A mice consistent with more hydroxylated lysines at helical cross-linking sites (N = 5, p<0.02). The percentage hydroxylation of α1(I) chain N- and C- telopeptides from mass spectrometry appeared not to be affected (N = 1, both genotypes, p = NS).