The 3'-5' exoribonuclease ISG20L2 contributes to 3' terminus maturation of 18S and 28S ribosomal RNAs

Ribosome biogenesis is a nodal process in cell growth and proliferation, and its dysfunction is associated to human congenital diseases and tumorigenesis. Despite extensive mechanistic characterization, several processing steps of human pre-ribosomal RNAs remain elusive. The 47S primary transcript contains 3 out of 4 ribosomal RNAs flanked by external (5'ETS, 3'ETS) and internal (ITS1, ITS2) transcribed spacers. The molecular processes leading to the removal of the 3'ETS, one of the earliest maturation steps, is not fully understood. Combining loss-of-function experiments and 3'-RACE high-throughput sequencing, we showed that the vertebrate-specific 3'-5' exoribonuclease ISG20L2, a DEDDh RNase T superfamily member, is critical for efficient removal of the 3'ETS and formation of large ribosomal subunits. ISG20L2 inactivation led to accumulation of various forms of 3'-extented pre-rRNAs and disorganized PDFC, a sub-nucleolar compartment recently linked to 3'ETS processing. The function of ISG20L2 also extends to the trimming of ITS1 after endonucleolytic cleavage at site 2 and its knockdown reveals the precise location of site 2. Altogether, the present work uncovers the landscape of two pre-rRNA processing steps at nucleotide resolution, and restricts the function of ISG20L2 to the nucleoli where it contributes to the maturation of 18S and 28S 3' ends. Overall design: HeLa cells were treated with a control siRNA duplex (siRNA scramble) ou siRNA duplexes targeting ISG20L2 mRNA.