Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing [dCas9-DNMT3A ChIP-seq]

To investigate the genome-wide occupancy of the DNA methylation editing tool dCas9-DNMT3A in cells harboring non-targeting sgRNAs (CTRL) and cells harboring sgRNAs targeting the IL1RN promoter (sgIL1RN). We analyzed the occupancy of dCas9-DNMT3A using chromatin immunoprecipitation and high-throughput sequencing (ChIP-seq). We compared IL1RN methylation-edited cells (sgIL1RN) with non-edited cells (CTRL) using biological duplicates.