Comparative transcriptomic analysis between wild-type (WT) and Cables2-null embryos.

We found that conventional targeted disruption of the entire Cables2 locus caused post-gastrulation embryonic lethality in mouse. To examine global gene changes during gastrulation, we performed the RNA-seq experiment using WT- and Cables2-null embryos at embryonic day 6.5 and 7.5. mRNA profiles of WT- and Cables2-null mouse embryos at E6.5 and E7.5我们发现，对小鼠的Cables2基因座（Cables2 locus）进行完整的传统靶向敲除，会导致小鼠胚胎在原肠胚形成后出现致死现象。为探究原肠胚形成过程中的全基因组基因表达变化，我们针对胚胎发育第6.5天（E6.5）与第7.5天（E7.5）的野生型（Wild Type）及Cables2基因敲除（Cables2-null）小鼠胚胎开展了RNA测序（RNA-seq）实验。本数据集包含上述两个发育阶段野生型与Cables2基因敲除小鼠胚胎的mRNA表达谱。