Viral entry is affected by mutations in the hexameric interface.
收藏Figshare2016-02-23 更新2026-04-29 收录
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(A) Relative entry levels of NiV/VSV-rluc virions containing wt NiV-G and wt NiV-F (solid black line) or mutant L53D, V108D, R109L or Q393L NiV-F (dotted red line). Vector alone (pcDNA3)/VSV is shown as a dotted gray line. RLU for lysates of infected Vero cells were quantified 18–24 h post infection and plotted against the number of viral genomes/ml over 3 logs of viral input. Data shown are averages ± SEM from at least three independent experiments (n = 3). (B) Representative Western blot analysis of NiV/VSV-rluc virions shown under Fig 5A. 4x108 NiV/VSV pseudotyped virions (genome copies) were separated by denaturing 10% SDS–PAGE and probed against NiV-G (rabbit anti-HA, Bethyl) and NiV-F (mouse anti-AU1, Covance).
创建时间：
2016-02-23
