Colocalization coefficients of internal FH in HUVECs concurrently stained with VWF, β-actin and FI.

Colocalization coefficients, Pearson’s (PCC) and Manders’ (M1 and M2), were measured in HUVEC images stained concurrently for FH and with VWF, β-actin, or FI, using the antibody pairs 2, 3 and 4 described under Internal HUVEC FH, VWF, β-actin and FI Detection in the Methods section. Data were analyzed in 2–4 images per experiment from 4–7 experiments with each antibody pair.aIndicates signal correlation in the two channels.Colocalization coefficients of internal FH in HUVECs concurrently stained with VWF, β-actin and FI.