Anti-cancer activity of Cotyledon orbiculata extract

We investigated the effects of the crude extract of a South African medicinal plant, Cotyledon orbiculata, on cell survival of colon (HCT116) cancer cell lines. Using RNASeq, we discovered that the extract interfered with mRNA regulatory pathways. Here, we found that the extract of Cotyledon orbiculata, a South African medicinal plant, had an anti-proliferative effect in cancer cells, mediated by apoptosis induced by alternative splicing of hnRNPA2B1 and BCL2L1. Overall design: RNA sequencing was used to determine the effect of crude extract from the Cotyledone orbiculata on differential gene expression and splicing in human colon cancer cells, relative untreated control cells.