Global transcriptomic portrait of biofilm lifestyle in the clincial isolate of Candida glabrata

Aim: of the study was to compare the transcriptomic profiling of planktonic and biofilm growth phase of clinical isolate of Candida glabrata Methodology: RNA was extracted using Hi-PurA yeast RNA purification kit from biofilm and planktonic growth phase and sequenced by Illumina Hi-Seq 2500 platform with 250bp paired chemistry Results: we got 959 significantly Differential Expressed Genes DEGs (logFC => 1.5) acconts for 18.11 % in which 596 (11.26 %) are getting downregulated and 363 (6.85%) upregulated in the biofilm growth mode. We validated 8 genes by qPCR Conclusion: Comprehensive analysis of the large expression gene sets generated from experimental conditions (Planktonic vs Biofilm) by RNA Seq revealed the unique network of genes associated with this intricate and complex process. We observed that Differentially Expressed Genes (DEGs) associated with Glyoxylate and Dicarboxylate metabolism, Carbon-Carbon lyase activity Overall design: mRNA of 24h grown biofilm growth phase from 3 biological replicates and similarly 3 biological replicates were taken from planktonic growth phase