SWATH-Proteomics of Mathurameha in High Glucose-Induced Endothelial Dysfunction

This study aimed to elucidate the mechanisms underlying the anti-diabetic effects of Mathurameha against high glucose-induced endothelial dysfunction by SWATH-MS. The EA.hy926 cells were treated with normal glucose, high glucose, or high glucose plus Mathurameha for 24 h. Proteins were extracted and resolved on 12% SDS-PAGE. Coomassie blue-stained gel were excised in the whole lane and subjected to in-gel tryptic digestion and SWATH-MS.