Figure S1 - Inhibiting HER3-Mediated Tumor Cell Growth with Affibody Molecules Engineered to Low Picomolar Affinity by Position-Directed Error-Prone PCR-Like Diversification

Theoretical binomial distribution of mutation frequencies in the library and theoretical calculations on combinatorics and library coverage. A. Distribution of mutation frequencies in a library that is randomized in 13 positions with a mutation frequency of 23% in each position (3/13), assuming that the incorporation of codons follow a binomial distribution. B. Theoretical calculation of the number of combinations of 3 mutations per molecule in a library design that is intended to randomize 13 positions with 16 different codons and a mutation frequency of 23% in each position (3/13).
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