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Detection of SARS-CoV by Real-Time Quantitative PCR in Spiked Human Blood Samples

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/SARS_Transmission_Pattern_in_Singapore_Reassessed_by_Viral_Sequence_Variation_Analysis/153257
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The x-axis denotes the cycle number of the quantitative PCR assay, and the y-axis denotes fluorescence intensity (F2) over the background level. RNA standards were as follows: 1.05 × 106 copies per reaction (line a), 1.01 × 105 copies per reaction (line b), 9.4 × 103 copies per reaction (line c), 8.9 × 102 copies per reaction (line d), and 1.07 × 102 copies per reaction (line e). The virus loads determined in the five spiked human blood samples were as follows: SB1, 1.64 × 106 copies per reaction; SB2, 3.84 × 103 copies per reaction; SB3, 2.17 × 103 copies per reaction; SB4, 6.21 × 102 copies per reaction; and SB5, 1.20 × 102 copies per reaction. NTC, non-template control. (244 KB DOC).
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2015-12-02
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