S4E Figure

S4 Fig. IFN-β induction during KSHV lytic reactivation requires all three IFN-β enhanceosome transcription factors. BC-3 cells with stably integrated tdTomato reporters driven by the promoters carrying mutations in the binding sites for IRF3, NF-κB, AP-1 were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”) and 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”). Cells were stained for KSHV ORF45 and analyzed by flow cytometry. ns = p>0.05, *** = p<0.001, **** = p<0.0001. Two-Way ANOVA followed by Dunnett’s post hoc multiple comparisons test for all experiments. n= 4.