Transcriptional responses to TNF following HFol treatment of wild type and GCN2 depleted synoviocytes

We report the effects of Hfol on TNF induction of inflammatory genes in wild type cells versus cells depleted of GCN2 RNA-seq analysis of wild type or GCN2-depleted established fibroblast like synoviocytes (K4 cells) treated with TNFa in the presence or absence of Halofuginone (HF). To test the requirement for the protein kinase GCN2 in the effects of the aminoacyl tRNA synthetase (AARS) inhibitor HF, wild type K4 cells or cells depleted of GCN2 by stable infection with a lentiviral shRNA directed against GCN2 cells in culture were treated, using duplicate samples for each condition, for 16 hours with HF and 8 hours with TNFa.