figures for erythrocyte traits and platelets.pdf

<b>Erythrocyte traits results</b> The GWAS results is shown in Table1. And the results are also visualrized by Manhattan plots and corresponding quantile-quantile plots(QQ-plots), which are shown in Figure1 and Figure2. We performed a genome-wide association study for six traits of erythrocyte traits: red blood cell(RBC),mean corpuscular haemoglobin(MCH),mean corpuscular haemoglobin concentration(MCHC),mean corpuscular volume(MCV),haematocrit(HCT),and haemoglobin(HGB). After analysed by Farm-CPU, there are in total twenty three significant SNPs associated with erythrocyte traits: six for RBC, two for MCH, seven for MCHC, two for MCV, four for HCT and two for HGB.Three of these twenty-three SNPs fall into the regions that related with hepatoma (liver cancer), carcinoma of the rectum (colorectal cancer) and cancer, which are harboured within regions of known genes. Other two SNPs fall into regions that have been reported to harbour QTL for Alzheimer’s disease and regulating and controlling of hemameba (WBC) and phagocyte, which fall into regions of known genes. Others are located 11157 to 89626bp away from the nearest known genes. All SNPs are not assigned to any chromosome but all significant SNPs reached genome-wide level. We find KCNJ2 as an candidate gene meanwhile the SNP (BOVINEHD1900016940) on Bos taurus chromosome (BTA)19 was located in the known gene(KCNJ2), and 13Mbp away nearby the gene, which was highly associated with RBC(p= 1.93E-07). <b>Platelet traits results</b> The GWAS study for platelet contain five traits: platelet count(PLT),platelet distribution width(PDW),mean platelet volume(MPV),large platelet count ratio(LPCR),platelet distribution wide ratio(PDWR).Twenty-seven significant SNPs were detected to be associated with five platelet traits: none for PLT, five for PDW, eleven for MPV, five for LPCR, five for PDWR. The association of these 27 highly significant SNP loci with immunity, cancer occurrence, cancer suppression and health-related traits has not been clearly verified. Moreover, among these 27 significant SNPs, 10SNPs are harboured within regions of nearest known genes, these SNPs are located from 2709 to 621763 bp away from the nearest genes. But none is assigned to any chromosome. And the GWAS results are shown in Table2. Results are also visualrized via Manhattan plots and corresponding quantile-quantile plots(QQ-plots), which are shown in Figure3 and Figure4.