scRNA-seq of CD45+ cells from salivary gland of Aire-knockout rats

This dataset examines the impact of anti-IFNα autoantibodies on immune
cell populations and gene expression in peripheral immune tissues of
Aire-knockout rats. Single-cell RNA sequencing (scRNA-seq) was performed
on CD45+ cells sorted from the submandibular salivary glands of
seven-month-old Aire-deficient rats (n=4) and age-matched
Aire-heterozygous control rats (n=3). The salivary glands were minced,
enzymatically digested, and processed to avoid cell aggregation before
sorting for CD45+ cells. The single cells were captured using the 10x
Chromium platform, with cDNA libraries prepared using the single-cell 3’
mRNA kit. Sequencing was conducted on the Illumina MiSeq platform, and the
data were mapped to the Rattus norvegicus reference genome (Rnor_6.0) and
processed with the Cell Ranger software suite. The dataset includes count
matrices generated by Cell Ranger.