Genome-wide maps of upregulated or downregulated genes in reconstituted SW48O KOEMT cells without or with a nuclear PTEN (C124S) mutant. Genome-wide maps of upregulated or downregulated genes in reconstituted SW48O KOEMT cells without or with a nuclear PTEN (C124S) mutant

We report that nuclear PTEN negatively regulates DNA-binding activity of SMAD3. We compared upregulated or downregulated genes in colorectal tumor cells without or with a nuclear PTEN (C124S) mutant. Overall design: RNA-seq analysis of upregulated or downregulated genes in colorectal tumor cells SW480, SW480 with PTEN knockout (SW480 KOEMT), and PTEN-negatvie SW480 reconstituted with a nuclear PTEN (C124S) mutant (SW480 KOEMT + nuclear PTEN [C124S]).