Detection of LAM by the Uri-TB-direct test in urine of patients with microbiologically verified TB.

Results in optical density (OD) at absorbance 450 n.* Sputum samples were liquefied and decontaminated using N-acetyl-L-cysteine and NaOH (final concentration 1.5%). Portions of the decontaminated pellet were used for microscopy, culture and PCR. Smear microscopy was performed using auramine staining. For culture the material was inoculated into a Mycobacteria Growth Indicator Tube (MGIT) (Becton Dickinson, USA) and solid Lowenstein Jensen medium and incubated for up to 42 days. The Cobas TaqMan MTB test (Roche Diagnostics, Basel, Switzerland) was used for PCR.** Not doneDetection of LAM by the Uri-TB-direct test in urine of patients with microbiologically verified TB.