FACS-isolated Lin-CD24-CD49f-Sca-1+stromal cells from adult mouse proximal and distal prostatic ducts

We have noticed that the proliferative potential of epithelial cells in the mouse proximal prostatic ducts is less than those at the distal prostatic ducts. We were curious whether the tissue microenvironment in the two anatomic regions play a role in the distinct epithelial proliferative indices. So we isolated respective stromal cells in the two regions and performed an RNA-seq analysis. To identify such signaling, we performed an RNA sequencing(RNA-seq) analysis using fluorescence-activated cell sorting(FACS)-isolated Lin-CD24-CD49f-Sca-1+stromal cells from adult mouse proximal and distal prostatic ducts