Ribo-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult mice

We carried out ribosome footprinting on the Quadriceps femoris muscle of 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated. The treatments were started at the age of 15 months. The main goal of the study was to decipher the translation changes in wild-type aging and upon above mentioned anti-aging treatments. We further investigated non-canonical targets of translational control and identify novel translational units.本研究针对四组小鼠的股四头肌（Quadriceps femoris）开展核糖体足迹实验（ribosome footprinting），各组样本分别为10月龄野生型小鼠、30月龄野生型小鼠、自15月龄起实施热量限制的30月龄小鼠，以及自15月龄起接受雷帕霉素（rapamycin）处理的30月龄小鼠。本研究的核心目标为解析野生型衰老进程以及上述抗衰老处理下的翻译调控变化；同时，本研究对翻译调控的非经典靶点展开探究，并鉴定出新型翻译单元。