Transcriptomic analysis of pediatric T-cell acute lymphoblastic leukemia

The aim of this study was to investigate the transcriptome of 35 primary samples of pediatric patients diagnosed with T-cell acute lymphoblastic leukemia and 6 samples of healthy T-cell precursor subsets from pediatric donors (3 samples of CD34+ and 3 samples of CD4+ CD8+ thymic subsets). Samples were collected before treatment. Sequencing libraries were obtained with Illumina TruSeq Stranded mRNA protocol, with modified conditions of RNA fragmentation (90°C for 2 minutes). All libraries were sequenced on Illumina NovaSeq6000 platform, in 2x150PE mode (paired end sequencing with 150 nt reads), with a coverage of 150M reads/sample.