Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus. Transcriptomic analysis of leaves and leaf cells from the medicinal plant Catharanthus roseus

The goal of this study is to investigate differential transcription profiles of leaf material/cells accumulating different levels of alkaloids in the anticancer plant Catharanthus roseus. Overall design: Gene expression profiling was performed for three biological replicates of: i) protoplasts of specific leaf cells specialized in alkaloid accumulation (idioblasts) and their control mesophyll protoplasts, total leaf protoplasts, and origin leaves; ii) 4 different developmental stages of leaves from plants growing in two different conditions leading to differential accumulation of alkaloids.