DataSheet_2_Prevalence and antibiotic resistance of Staphylococcus aureus associated with a college-aged cohort: life-style factors that contribute to nasal carriage.xlsx

Staphylococcus aureus is an opportunistic human pathogen that can frequently be found at various body locations, such as the upper respiratory tract, nostrils, skin, and perineum. S. aureus is responsible for causing a variety of conditions, which range from minor skin infections and food poisoning to life-threatening sepsis and endocarditis. Furthermore, S. aureus has developed resistance to numerous antimicrobial agents, which has made treatment of S. aureus infections difficult. In the present study, we examined lifestyle factors that could increase the likelihood of S. aureus carriage, the overall prevalence of S. aureus, as well as assessed the antibiotic resistance profiles of the S. aureus isolates among a population of college students. Five hundred nasal samples were collected and analyzed via selective growth media, coagulase and protein A testing, as well as polymerase chain reaction and DNA sequencing. One hundred four out of the 500 samples collected (21%) were identified as containing S. aureus. The S. aureus isolates were resistant to penicillin (74%), azithromycin (34%), cefoxitin (5%), ciprofloxacin (5%), tetracycline (4%), and trimethoprim (1%), but sensitive to gentamicin and rifampin. Lastly, we identified several lifestyle factors (i.e., pet exposure, time spent at the university recreational facility, musical instrument usage, and tobacco usage) positively correlated with S. aureus nasal colonization.

金黄色葡萄球菌作为一种机会性人类病原体，常存在于人体的多个部位，诸如上呼吸道、鼻孔、皮肤及会阴等。金黄色葡萄球菌可引发多种病症，其严重程度从轻微的皮肤感染和食物中毒，到危及生命的败血症和心内膜炎不等。此外，金黄色葡萄球菌对多种抗菌药物产生了耐药性，这使得金黄色葡萄球菌感染的诊治变得尤为棘手。在本研究中，我们探讨了可能增加金黄色葡萄球菌携带几率的日常生活因素，以及金黄色葡萄球菌的总体携带率，并对大学生群体中金黄色葡萄球菌分离株的抗生素耐药性进行了评估。共收集并分析了500份鼻拭子样本，通过选择性培养基培养、凝固酶和蛋白A测试、聚合酶链式反应及DNA测序等方法进行检测。在收集的500份样本中，有104份（占21%）被确认为含有金黄色葡萄球菌。金黄色葡萄球菌分离株对青霉素（74%）、阿奇霉素（34%）、头孢西丁（5%）、环丙沙星（5%）、四环素（4%）和甲氧苄啶（1%）耐药，但对庆大霉素和利福平敏感。最后，我们识别出几种与金黄色葡萄球菌鼻部定植呈正相关的日常生活因素，包括宠物接触、在大学娱乐设施停留时间、乐器使用及烟草使用等。