Enhanced DNA repair and genomic stability identify a novel HIV related Diffuse Large B-cell Lymphoma subtype [aCGH]

Transcriptional and genomic profiling study of HIV positive and HIV negative Diffuse Large B-Cell Lymphoma (DLBCL). Tumors were subtyped using the Lymph2Cx assay. Only GCB (germinal center like B-cell) subtypes were then subjected to digitial gene expression profiling and array comparative genomic hybridization (aCGH). The study used DNA extracted from FFPE DLBCL tumors derived from HIV(+) and HIV(-) patients to assess copy number variation differences between the HIV(+) and HIV(-) cohorts. aCGH data was correlated with gene expression & IHC data. aCGH was performed on 19 HIV positive and 20 HIV negative GCB DLBCL samples using Agilent SurePrint G3 human CGH arrays. DLBCL is more agreesive in HIV(+) patients compared to HIV(-) individuals. Study objective was to identify novel markers and potential therapeutic targets that contribute to the enhanced agressive nature of this disease in HIV infected individuals.