transcriptional landscape of Germ cell differentiation at single cell resolution

Single cell sequencing technologies are powerful tools for the dissection of large regulatory networks and their role in directing developmental trajectories. The goal of this project is to profile the transcriptional landscape of germ cell development in the mouse male embryo at single cell resolution, and to examine the dynamic regulation of the molecular networks directing epigenetic reprogramming in these cells. Single cell RNAseq profiling (10x Genomics) of Primordial Germ Cells isolated via Fluorescence Actvated Cell Sorting, using a germ cell specific Oct4-EGFP fluorescent reporter. One replicate per time point (E10.5 and E13.5)单细胞测序技术（Single cell sequencing technologies）是解析大型调控网络及其在调控发育轨迹中发挥作用的强有力工具。本项目旨在以单细胞分辨率解析小鼠雄性胚胎生殖细胞发育的转录组全貌，并探究介导这些细胞表观遗传重编程（epigenetic reprogramming）的分子网络的动态调控机制。本研究采用生殖细胞特异性Oct4-EGFP荧光报告基因，通过荧光激活细胞分选（Fluorescence Activated Cell Sorting）分离原始生殖细胞（Primordial Germ Cells），并借助10x基因组学（10x Genomics）平台完成单细胞RNA测序（Single cell RNAseq）分析。每个时间点（E10.5与E13.5）设置一个生物学重复。