Expression data from murine glioma stem cells treated with or without doranidazole under normoxic or hypoxic conditions

Under hypoxic conditions, nitroimidazole compounds accumulate in cells in their reduced form and have oxygen-mimetic effects, serving as markers of hypoxia and radiosensitizers. The full potential of their bioreductive metabolism, including cytotoxicity for cancer stem cells, has not been sufficiently explored, however. Here we investigated the changes in gene expression induced by treatment with 2-nitroimidazole doranidazole in murine glioma stem cells, under normoxic or hypoxic conditions. Murine glioma stem cells (GSCs) were treated with PBS (control) or 3 mM doranidazole under normoxic conditions (21%O2) or hypoxic (1%O2) conditions for 24 h. Three independent replicates were analyzed for each goup. Gene expression was measured with Clariom S Mouse Arrays.