Zfhx3 is required for the differentiation of late born D1-type medium spiny neurons

Purpose: The goals of this study are to compare the LGE-derived cells changes gene expression profiles between controls and Zfhx3 conditional knockout mice Methods: Total RNA was isolated and sequenced using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered significantly changed whicn perform P value <= 0.05 Results: Compared to controls, 1620 genes were significantly down regulated, and 2108 genes were up regulated in the RNA expression level of the mutant LGE. LGE RNA profiles of E16.5 control mouse brain and Zfhx3 conditional knockout mouse brain研究目的：本研究旨在对比对照组与Zfhx3条件性敲除小鼠的LGE（Lateral Ganglionic Eminence）来源细胞的基因表达谱差异。 研究方法：提取总RNA后使用Illumina HiSeq 2500进行测序；原始数据通过TopHat进行分析；当P值≤0.05时，判定基因表达存在显著变化。 研究结果：与对照组相比，突变型LGE的RNA表达水平中，共有1620个基因显著下调，2108个基因显著上调。本研究检测了E16.5胎龄对照组小鼠脑组织与Zfhx3条件性敲除小鼠脑组织的LGE RNA表达谱。