Single-cell RNAseq profiling of HeLa cells after stimulation with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)

HeLa cells were treated with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL: 10, 25 or 40ng/ml) or with the drug vehicle ("Control"), for 50 minutes or 2 hours prior to analyses by single-cell RNA sequencing. The objective was to study the extent of gene expression variability in resting cells and to evaluate the effect of time and dose of TRAIL treatments on gene expression variability. HeLa cells from the different conditions were processed following standard cell hashing protocol with Hashtag oligonucleotide (HTO), pooled, and single cell RNAseq profiling was performed using the 10x Genomics single-cell 3' V2 chemistry. Correspondence between the Hashtags and conditions was: A0251: control; A0252: Trail 25ng/ml for 50mn; A0253: Trail 10ng/ml for 120mn; A0254: Trail 25ng/ml for 120mn; A0255: Trail 40ng/ml for 120mn.