Transcriptome profiling of rat brain samples in two age groups in responding to intracerebral hemorrhage

Aging-induced decline of endogenous neuroprotection has been shown to aggravate intracerebral hemorrhage (ICH)-induced acute brain injury, however, the underlying mechanisms in brain are still little known. In this study, we applied a rat ICH model to study the transcriptional responses in the early and late aging (13-month and 22-month old) rats that show substantial differences in brain damage and recovery. Transcriptome analysis (RNA-seq) reveals that brain expression of neuroinflammation genes is similarly and selectively upregulated in ICH, which includes genes in the cellular response to interferon gamma function. We show that the anti-IFN-γ treatment effectively reduces ICH-induced acute brain injury.衰老诱导的内源性神经保护（endogenous neuroprotection）功能衰退，已被证实会加重脑出血（intracerebral hemorrhage, ICH）引发的急性脑损伤，但目前脑内的潜在作用机制仍不甚明晰。本研究采用大鼠脑出血模型，针对脑损伤与恢复程度存在显著差异的早期衰老（13月龄）和晚期衰老（22月龄）大鼠，探究其脑组织的转录组响应特征。转录组分析（RNA测序，RNA-seq）结果显示，脑出血模型大鼠脑内的神经炎症相关基因呈现相似且选择性的上调表达，其中涵盖参与干扰素γ（interferon gamma, IFN-γ）细胞应答过程的功能基因。本研究证实，抗干扰素γ（anti-IFN-γ）干预可有效减轻脑出血诱导的急性脑损伤。