Whole-transcriptome microarray to identify sexually dimorphic RNA transcripts that are differentially expressed in disease-susceptible versus nonsusceptible CNS regions of male and female SJL mice

We hypothesized that sexual dimorphism in experimental autoimmune enecphalomyelitis arises from sexually dimorphic and CNS-region specific expression of genes that regulate BBB permeability and leukocyte entry. To test this hypothesis, we profiled total RNA from the cerebella and frontal cortices of naive female versus male SJL mice 100 ng of total RNA was extracted from cerebella and frontal cortices of naive SJL female and male littermates to identify differential expression of gene transcripts unique to female cerebellum.我们提出如下假说：实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis）中的性别二态性，源于调控血脑屏障（Blood-Brain Barrier, BBB）通透性与白细胞浸润的基因，其表达兼具性别二态性与中枢神经系统（Central Nervous System, CNS）区域特异性。为验证该假说，我们对未致敏SJL品系雌性与雄性小鼠的小脑与额叶皮层总RNA开展了表达谱分析；具体而言，我们从同窝未致敏SJL雌雄小鼠的小脑与额叶皮层中提取100 ng总RNA，旨在鉴定雌性小脑所特有的基因转录本的差异表达情况。