Peptidoglycan differentially cross-tolerises P. gingivalis-stimulated CD14hi/lo M1 and M2 MΦ subsets.

CD14hi/lo M1 and M2 MΦ subsets exhibit a differential cross-tolerisation of cytokine production and NFκB activity to the P gingivalis PAMPs, PG-LPS and HKPG. CD14lo M1, CD14hi M1, CD14lo M2 and CD14hi M2 MΦ subsets were pre-stimulated (tolerised) with 10µg/ml peptidoglycan (PGN) for 24 hours prior to stimulation with PG-LPS or HKPG and incubated for a further 18 hours. Tolerisation/suppression of the pro-inflammatory cytokines (TNFα, IL-1β and IL-6), the anti-inflammatory cytokine (IL-10) and the transcription factor activity (NFκB) is expressed as the mean percentage suppression ± SD of non-tolerised stimulation controls. Data displayed represents triplicate samples for n = 3 replicate experiments.