1) Transcriptional profiling analysis of NLP7 regulated genes in Arabidopsis. 2) Transcriptional profiling analysis of Ca2+ regulated genes in Arabidopsis. 3) ChIP-seq and Genome-wide identification of NLP7 targets in Arabidopsis.

1) To determine how the transcription factor NLP7 regulates plant responses to cold stress, cold regulated transcriptome in wild type and nlp7-1 mutant was compared.2) To elucidate the function of Ca2+ in the context of cold stress, cold regulated transcriptome in wild-type plants pretreated or not with EGTA was compared.3) To identify direct targets of NLP7 globally, we performed ChIP-seq using NLP7pro:NLP7-GFP transgenic seedlings.