MouseLight Neuron AA1627

A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions. Inside the mouse brain, individual neurons zigzag across hemispheres, embroider branching patterns and, researchers have now discovered, can even spool out spindly fibers up to 45 centimeters long. Scientists can see and explore these wandering neural traces in 3-D in the most detailed map of mouse brain wiring yet created. The map reconstructs the shape and position of more than 300 of the 70 million neurons in the mouse brain. Previous efforts to trace the path of individual neurons had topped out in the dozens. The selectively-labeled neurons were mapped in an iterative process with two-photon microscopy. The brain is sliced in 200-micron sections and a few dozen neurons are labeled at a time and imaged. Each brain imaged yields about 50 terabytes of data, each containing mapped neurons which can be browsed via the NeuronBrowser application at https://ml-neuronbrowser.janelia.org/. Researchers interested in the complete raw data set should contact Janelia to discuss obtaining it via hardware transfer.

由Janelia科研团队发起的一项庞大的神经网络追踪工作，将小鼠大脑中完全追踪的神经元数量提升了十倍。研究人员现在可以下载并浏览三维空间中的数据。在小鼠大脑内部，单个神经元穿梭于半球之间，编织出分支图案，研究人员最新发现，这些神经元甚至可以延伸出长达45厘米的细长纤维。科学家们能够通过迄今为止创建的最详尽的小鼠大脑接线图，在三维空间中观察并探索这些游走神经元的轨迹。该图重建了小鼠大脑中7000万神经元中的300多个的形状和位置。先前追踪单个神经元路径的努力最多只能追踪到数十个。通过迭代过程和双光子显微镜，对选择性标记的神经元进行了绘制。大脑被切成200微米厚的切片，每次标记和成像的神经元数量仅有数十个。每张成像的大脑会产生约50太字节的数据，其中包含的绘制神经元可通过NeuronBrowser应用程序进行浏览，该应用程序的网址为https://ml-neuronbrowser.janelia.org/。对完整原始数据集感兴趣的研究人员应联系Janelia，以讨论通过硬件传输获取数据的方式。