Next generation sequencing of parental RM cells and RM1 cells derived bone and lung metastases for preliminary assessment of differential tumor cell gene expression in bone

Using the immunocompetent bone-metastatic murine model of prostate cancer, the transcriptomes of proliferating (PKH26-) eCFP+/luc2+ RM1 cells (referred to as RM1536) were compared to FACS-isolated, proliferating (PKH26-) RM1536 cells derived from matched bone and lung metastases for preliminary assessment of gene alterations that may specifically occur in bone. mRNA profiles of unlabelled RM1 cells (n = 2), eCFP+/luc2+ RM1 (RM1536; n = 2), proliferating (PKH-) bone-derived RM1536 (n = 1) and proliferating (PKH-) lung-derived RM1536 (n = 1) from the same animal at D16 post-intracardiac injection of PKH26-labelled tumor cells were compared following read generation using the Illumina HiSeq2500 platform.