Integrated single-cell analysis of preleukemic mouse models

Hematological malignancies develop through acquisition of mutations and clonal expansion in hematopoietic stem and progenitor cells (HSPCs). However, how individual mutations perturb the entire hematopoietic system towards malignant phenotypes remains elusive. Here, we have performed single cell RNA sequencing of bone marrow HSPCs from 8 different mutant mouse models to study mutation-specific preleukemic perturbations. Overall design: Single cell RNA sequencing of lineage negative / c-Kit positive (LK) cells for 38 animals from eight different mutant mouse models and their wild-type littermates