Comparison of Y-142 and Y-073 binding activities toward proHB-EGF.

A. Binding of anti-HB-EGF antibody to proHB-EGF. The cells were incubated with anti-HB-EGF antibody, followed by incubation with Alexa488-labeled anti-mouse IgG antibody. The binding was detected by flow cytometry. No IgG, black line; Y-142, black histogram; Y-073, gray histogram. B. Expression of amphiregulin (ARG) in cancer cell lines. Cells were incubated with anti-ARG antibody, followed by incubation with Alexa488-labeled anti-mouse IgG antibody. The binding activity of anti-ARG antibody was confirmed using EFO-27 cells transfected with an ARG expression plasmid. No IgG, black line; anti-ARG antibody, gray histogram.