comparison of gene expression profiles between single cloned mouse blastocysts (CBX109). unidentified

In an effort to gain further insights into reprogramming and the properties of SECNT embryos, we conducted large-scale gene expression profiling of single blastocysts using GeneChip microarrays. Sertoli cells, cumulus cells and embryonic stem cells were used as donor 1 - cells. In total 87 blastocysts were subject to microarray analysis.Using principal component analysis and hierarchical clustering, the gene expression profiles were clearly classified into three clusters corresponding to each donor cell type. The results revealed unique gene expression profiles for each type of SECNT embryo that were strictly dependent upon the type of donor cells, although considerable variation among individual profiles was observed within each group. This suggests that a specific reprogramming process occurs in each cloned embryo. Furthermore, on the basis of comparison analysis, we determined a set of genes, which were ?ghardly?h reprogrammed, and then we demonstrated that some of these genes were appropriately reprogrammed only in embryos that would acquire higher developmental competence. We provide a framework to further understand reprogramming in SECNT embryos.