Single cell sequencing of BMP4 stimulated enteroendocrine cells in organoids

Enteroendocrine cells (EECs) alter hormone expression while migrating along the crypt-villus axis. We report this swich is regulated by a BMP morphogen gradient. Single cell RNA sequencing of control and BMP4 stimulated EECs was performed using organoids from different hormone reporter mice. We show that BMP4 stimulation can switch different subsets of EECs from their crypt to villus state. We generated transciptome data from cells sorted from different hormone reporter mouse organoid lines with and without BMP4 stimulation, at different timepoints