Bioinformatics Analysis of Degenerative Meniscus Tissue in Knee Osteoarthritis of Wuzhishan Pig

Meniscus degeneration is one of the manifestations of knee osteoarthritis, but its specific molecular mechanism is still not very clear. We used Wuzhishan pig's anterior cruciate ligament resection to prepare meniscus degeneration models, and applied gene chip technology to detect the expression of differential genes in the degenerated meniscus tissue. The study detected a total of 893 differentially expressed genes, mainly related to hormones, apoptosis, inflammation and other mechanisms, and analyzed that TRP channels may play a key role. All in all, we have established a reliable animal model of meniscus degeneration and found that meniscus degeneration involves several possible molecular mechanisms, which provide molecular targets for future treatment of the disease. A total of 14 healthy adult male Hainan Wuzhishan pigs (Institute of animal husbandry and veterinary medicine, Hainan Academy of Agricultural Sciences, China), weighing 15-18kg, aged from 6 to 7 months, were not raised in single cage, and the environmental temperature was 20-28℃. The experimental animals were randomly divided into control group (sham group) and experimental group (OA group), normal light and diet.