Identification and control for the effects of bioinformatic globin depletion on human RNA- seq differential expression analysis

To compare the impact of haemoglobin depletion by hybridisation-based removal or bioinformatic removal of reads on the outcome of differential gene expression analysis. This comparisons was performed using RNA-seq data from 58 human tuberculosis (TB) patient whole blood samples – 29 globin kit-depleted and 29 matched non-depleted – a subset of which were taken at TB diagnosis and at six months post-TB treatment from the same patient Comparative gene expression profiling analysis of whole blood RNA-seq data from TB patients before and after treatment and quantitative assessment of the effects of kit depletion or bioinformatic depletion on this outcome.