Data from: Shared and unique microbes between Small hive beetles (Aethina tumida) and their honey bee hosts

The small hive beetle (SHB) Aethina tumida is an opportunistic parasite that feeds on bee larvae, honey, and pollen. While SHBs can also feed on fruit and other plant products, like its plant‐feeding relatives, SHBs prefer to feed on hive resources and only reproduce inside bee colonies. As parasites, SHBs are inevitably exposed to bee‐associated microbes, either directly from the bees or from the hive environment. These microbes have unknown impacts on beetles, nor is it known how extensively beetles transfer microbes among their bee hosts. To identify sets of beetle microbes and the transmission of microbes from bees to beetles, a metagenomic analysis was performed. We identified sets of herbivore‐associated bacteria, as well as typical bee symbiotic bacteria for pollen digestion, in SHB larvae and adults. Deformed wing virus was highly abundant in beetles, which colonize SHBs as suggested by a controlled feeding trial. Our data suggest SHBs are vectors for pathogen transmission among bees and between colonies. The dispersal of host pathogens by social parasites via floral resources and the hive environment increases the threats of these parasites to honey bees.
DNA and RNA sequencing reads were previously deposited at NCBI‐BioProject PRJNA256171.
Supporting datasets provided in Excel spreadsheets comprise:

Microbes found from Illumina DNA reads using Kraken
DNA reads aligned to HoloBee Bar database report
Adult beetle microbe qPCR results from DNA extraction
Adult beetle de novo assembled contig_names

larvae beetle Pacbio_reads
Resources in this dataset:Resource Title: Website Pointer to Supporting Information. File Name: Web Page, url: https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.899#support-information-section Unmapped reads were used to screen microbial species with fully sequenced genome assemblies using Kraken with standard databases, which is designed to align short sequencing reads to sequenced microbe genomes.
-- Supporting Information S1, Microbes found from Illumina DNA reads using Kraken
mbo3899-sup-0001-SupInfoS1.xlsx. application/msexcel, 383.5 KB.

HoloBee‐Barcode uses a variety of markers as appropriate for each taxonomic group. Complete 16S ribosomal RNA was used for bacteria.
-- Supporting Information S2,DNA reads aligned to HoloBee Bar database report
mbo3899-sup-0002-SupInfoS2.xlsx. application/msexcel, 21.1 KB.
Detailed DNA extraction, RNA extraction, and qPCR protocols, along with the primers and results, are described in Supporting Information S3 and S4. Colonization by the fungus K. ohmeri on SHB adults was verified.
-- Supporting Information S3, Adult beetle microbe qPCR results from DNA extraction
mbo3899-sup-0003-SupInfoS3.xlsx application/msexcel, 52.2 KB.
The identified microbes show significant hit when blasting the assembled de novo contigs to Embl, Unigene, Est, Gss, Htc, Pat, Refseq, Htg, and Tst databases.
-- Supporting Information S4, Adult beetle de novo assembled contig_names; larvae beetle Pacbio_reads
mbo3899-sup-0004-SupInfoS4.xlsx application/msexcel, 4.1 MB.

小巢甲虫（SHB）Aethina tumida是一种机会主义寄生虫，以蜜蜂幼虫、蜂蜜和花粉为食。尽管SHB亦能摄食水果及其他植物产品，如同其植食性亲属，SHB仍偏好摄取蜂巢资源，并且仅在蜜蜂群体内繁殖。作为寄生虫，SHB不可避免地暴露于与蜜蜂相关的微生物中，这些微生物可能直接来自蜜蜂或蜂巢环境。这些微生物对甲虫的影响尚不明确，同样，甲虫之间如何广泛传播微生物亦未被认知。为识别甲虫微生物群体及其从蜜蜂传播至甲虫的微生物传播途径，我们进行了宏基因组分析。我们在SHB幼虫和成虫中识别出与食草动物相关的细菌群，以及典型的蜜蜂共生细菌，这些细菌有助于花粉的消化。变形翅病毒在甲虫中含量极高，如控制喂养试验所暗示，SHB可能成为病原体在蜜蜂之间以及蜂群之间传播的媒介。社会寄生虫通过花卉资源和蜂巢环境传播宿主病原体，增加了这些寄生虫对蜜蜂的威胁。

DNA和RNA测序读数先前已存档于NCBI-BioProject PRJNA256171。

支持数据集以Excel表格形式提供，包括以下内容：

* 使用Kraken从Illumina DNA读数中发现的微生物
* 定位至HoloBee Bar数据库报告的DNA读数
* 成虫甲虫微生物qPCR结果（来自DNA提取）
* 成虫甲虫从头组装的contig_names

幼虫甲虫Pacbio读数

本数据集包含的资源：
资源标题：指向支持信息的网站指针
文件名：网页
URL：https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.899#support-information-section
未映射读数被用于使用Kraken和标准数据库筛选完全测序的基因组组装的微生物物种，Kraken被设计为将短测序读数与测序微生物基因组对齐。

-- 支持信息S1：使用Kraken从Illumina DNA读数中发现的微生物
文件名：mbo3899-sup-0001-SupInfoS1.xlsx
MIME类型：application/msexcel，文件大小：383.5 KB。

-- 支持信息S2：定位至HoloBee Bar数据库报告的DNA读数
文件名：mbo3899-sup-0002-SupInfoS2.xlsx
MIME类型：application/msexcel，文件大小：21.1 KB。

-- 支持信息S3：成人甲虫微生物qPCR结果（来自DNA提取）
文件名：mbo3899-sup-0003-SupInfoS3.xlsx
MIME类型：application/msexcel，文件大小：52.2 KB。

-- 支持信息S4：成人甲虫从头组装的contig_names；幼虫甲虫Pacbio读数
文件名：mbo3899-sup-0004-SupInfoS4.xlsx
MIME类型：application/msexcel，文件大小：4.1 MB。

所识别的微生物在与Embl、Unigene、Est、Gss、Htc、Pat、Refseq、Htg和Tst数据库进行比对时显示出显著的匹配。