Genome-wide expression in asymmetric histone H3 mutants in Saccharomyces cerevisiae

Using histone H3 mutants designed so that they can only form a heterodimer, we studied the gene expression in a collection of mutants, comparing mutations to one histone H3 tail of a nucleosome compared to two histone H3 tails of a nucleosome.本研究通过设计仅能形成异二聚体（heterodimer）的组蛋白H3（histone H3）突变体，对一系列此类突变体的基因表达情况展开分析，并对比了核小体（nucleosome）中单个组蛋白H3尾部发生突变与两个组蛋白H3尾部发生突变的实验差异。